1981 etude de la synthse protrique in vivo dans le muscle de la truite arc en ciel salmo gairdneri r. Whole body protein turnover protein synthesis breakdown and net balance model enables quantification of the response to a variety of circumstances including the response to meal feeding.
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Whole body protein synthesis measurement. The rate of whole body protein synthesis g proteind was estimated from the total flux through the. The aim of the present work was to study whole body protein synthesis and breakdown as well as energy metabolism in very low birth weight premature infants 1500 g during their. Also by use of suitable arteriovenous sampling methods it is possible to make simultaneous measurements of synthesis not only by incorporation using tissue biopsies but also by. Influence of environmental tem perature in press. In contrast to the plateau observed with muscle protein synthesis whole body leucine oxidation a surrogate measure of protein oxidation increases in a linear fashion with graded protein intakes this linear relationship may be related to the combination of a relatively low k m for the rate controlling enzyme for leucine oxidation ie branched chain ketoacid dehydrogenase and a. The marked increase in whole body synthesis on feeding largely reflects the changes in protein synthesis in muscle which doubles on feeding compared with a 40 increase in that of the rest of the body.
The daily rates of synthesis of protein by the whole body and by the individual tissues were determined in two hereford x friesian heifers 236 kg and 263 kg live weight and a dry friesian cow 628 kg live weight. Abstract purpose of review in vivo estimation of protein turnover by stable isotopes in animals and humans has provided much relevant information on metabolic regulation and alterations for decades. While it was first appreciated at the whole body level in the 1970s and 1980s new approaches have allowed inter organ or tissue protein turnover rates to be measured notably the incorporation. Fauconneau b arnal m. In vivo protein synthesis in different tissues and the whole body of rainbow trout salmo gaidneri r. Tissue or secreted protein synthesis and whole body protein turnover can be calculated.
Underestimate whole body protein turnover to a variable extent with different amino acids 12 36. Rates of whole body protein synthesis and breakdown expressed as g proteinkg body weightday were significantly p less than 005 and positiviely correlated with per cent body surface area total burn per cent third degree burn and per cent open wound. Furthermore measurement of the rate of whole body protein synthesis from the specific activity of tissue amino acids at the end of the infusion may not provide a more reliable estima. Measurements have been made of whole body and skeletal muscle protein synthesis in fed and fasted adults with l 1 13 cleucine. In the fed state the whole body protein turnover model requires taking account of the contribution of absorbed tracee to the observed total appearance of tracee in the peripheral blood exogenous.
Gallery of Whole Body Protein Synthesis Measurement
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